Flow Cytometry systems rely on sophisticated fluid dynamics along with optical analysis to count and in some cases sort cell populations based on the fluorescence properties of the cells of interest. Cells labeled with fluorophores pass individually in a stream of fluid through an excitation beam causing the cells to fluoresce. The fluorescence signal is detected and the population of each fluorophore (and correspond tagged cell) is counted. In some systems the cells can then be sorted into groupings according to their fluorophore labels. The data generated by this technology can be used in diagnosis of health issues, medical research, and other areas of cell study such as micro-biology.
Flow cytometers leverage many of the same filters and principles used in fluorescence spectroscopy. Excitation filters can be used to ensure that only the desired wavelength is incident upon the sample flow. Emission filters are used in front of the detectors (typically photo-diodes) to clean-up the fluorescence signal, rejecting the excitation light and any undesirable light emitted by other fluorophores. A series of long and short pass dichroic filters are used at angles of incidence of 45° to direct the emitted signal to the detector corresponding to the colour of the fluorophore.
Iridian’s flow cytometry filters have high transmittance, steep edges, low ripple and deep blocking. We offer custom design and manufacture where required.
Further information on Iridian’s flow cytometry filters:Excitation (Laser Lines)
Dichroic – SPF
Dichroic – LPF
For custom flow cytometry filter solutions please contact us.